diff --git a/Mac/BuildScript/build-installer.py b/Mac/BuildScript/build-installer.py
index b789e30946a835..ff2b75c7d89131 100755
--- a/Mac/BuildScript/build-installer.py
+++ b/Mac/BuildScript/build-installer.py
@@ -1,4 +1,3 @@
-#!/usr/bin/env python
 """
 This script is used to build "official" universal installers on macOS.
 
diff --git a/Objects/typeslots.py b/Objects/typeslots.py
index 8ab05f91be12b0..41dc1c155fb1b4 100755
--- a/Objects/typeslots.py
+++ b/Objects/typeslots.py
@@ -1,5 +1,4 @@
-#!/usr/bin/python
-# Usage: typeslots.py < Include/typeslots.h typeslots.inc
+# Usage: ./python Objects/typeslots.py < Include/typeslots.h typeslots.inc
 
 import sys, re
 
diff --git a/Parser/asdl_c.py b/Parser/asdl_c.py
index 3e307610b635f4..85e730ef1242fb 100755
--- a/Parser/asdl_c.py
+++ b/Parser/asdl_c.py
@@ -1,4 +1,3 @@
-#! /usr/bin/env python
 """Generate C code from an ASDL description."""
 
 import sys
diff --git a/Python/makeopcodetargets.py b/Python/makeopcodetargets.py
index 33a4b4a76a1253..ac52c95ad45937 100755
--- a/Python/makeopcodetargets.py
+++ b/Python/makeopcodetargets.py
@@ -1,4 +1,3 @@
-#! /usr/bin/env python
 """Generate C code for the jump table of the threaded code interpreter
 (for compilers supporting computed gotos or "labels-as-values", such as gcc).
 """
diff --git a/Tools/clinic/clinic.py b/Tools/clinic/clinic.py
index 0ece814e8f1883..13b7710f9ca0f6 100755
--- a/Tools/clinic/clinic.py
+++ b/Tools/clinic/clinic.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3
-#
 # Argument Clinic
 # Copyright 2012-2013 by Larry Hastings.
 # Licensed to the PSF under a contributor agreement.
diff --git a/Tools/peg_generator/pegen/__main__.py b/Tools/peg_generator/pegen/__main__.py
index 2910d6ccf1c694..c7403422e1774b 100755
--- a/Tools/peg_generator/pegen/__main__.py
+++ b/Tools/peg_generator/pegen/__main__.py
@@ -1,5 +1,3 @@
-#!/usr/bin/env python3.8
-
 """pegen -- PEG Generator.
 
 Search the web for PEG Parsers for reference.